Oxidation-reduction potentials of the triphosphopyridine nucleotide system.

Oxidation-Reduction Potentials of the Triphosphopyridine Nucleotide Svstem*

Triphosphopyridine nucleotide serves as the primary hydrogen or electron acceptor in a variety of biological oxidations. Some dehydrogenases like glucose g-phosphate dehydrogenase (1) and isocitric acid dehydrogenase (2) have an absolute requirement for TPN. Other systems such as liver glutamic acid dehydrogenase (3, 4) can utilize either TPN or DPN about equally well. In addition, a large numb...

Studies on the biosynthesis of guanosine diphosphate L-fucose.

The kinetics of reduced triphosphopyridine nucleotide oxidation suggested that guanosine 5’.diphosphate-n-mamrose was converted to intermediates before reduction by reduced triphosphopyridine nucleotide (2). Subsequent studies revealed that the intermediates could be chemically reduced to guanosine 5’-diphosphate-6-deoxyhexoses (3). The present paper is concerned with the nature of the intermed...

The Stereospecificity of Thioredoxin Reductase for Triphosphopyridine Nucleotide.

Escherichia coli B contains a cytidine diphosphate reductase system which utilizes reduced triphosphopyridine nucleotide to effect the reduction of cytidine diphosphate to deoxycytidine diphosphate (1, 2). Two components, thioredoxin and thioredoxin reductase, have been identified as oxidation-reduction carriers between TPNH and cytidine diphosphate. Thioredoxin is a low molecular weight protei...

Studies on an Enzyme System from Wheat Germ Catalyzing the Aerobic Oxidation of Reduced Triphosphopyridine Nucleotide.

A triphosphopyridine nucleotide dependent alcohol dehydrogenase from Leuconostoc mesenteroides.

Ethanol formation during glucose fermentation is a relatively common activity of microorganisms; a few species, including Leuconostoc mesenterodes, produce ethanol as a major fermentation end product. The significant results from experiments with various species on this subject are: (1) at least three different metabolic pathways are available for anaerobic conversion of glucose to ethanol (Kos...